Western blot shows lysates of NTera-2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 1ug/ml of N1260-08B followed by Sheep IgG Secondary Antibody (HRP). A specific band was detected for Neuroligin 4X/NLGN4X at ~110kD (as indicated). This experiment was conducted under reducing conditions.
Neuroligin 4/NLGN4 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using N1260-08B at 5ug/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and their processes.
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